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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two more splice variants were reported but in-depth nucleotide sequence data is not nevertheless accessible.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide usually has the epitope regarded from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies sure to the blocking peptide not bind to the epitope to the target protein. By evaluating the staining with the blocked antibody versus the antibody by yourself, you can see which staining is specific.
Concentrate on Data Cyclin E belongs to the extremely conserved cyclin family, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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